Infectious bronchitis virus (IBV) affects poultry respiratory, renal and reproductive systems.Currently the efficacy Clothing - Junior Tops - Short Sleeve of available live attenuated or killed vaccines against IBV has been challenged.We designed a novel IBV vaccine alternative using a highly innovative platform called Self-Assembling Protein Nanoparticle (SAPN).In this vaccine, B cell epitopes derived from the second heptad repeat (HR2) region of IBV spike proteins were repetitively presented in its native trimeric conformation.In addition, flagellin was co-displayed in the SAPN to achieve a self-adjuvanted effect.
Three groups of chickens were immunized at four weeks of age with the vaccine prototype, IBV-Flagellin-SAPN, a negative-control Stoppers construct Flagellin-SAPN or a buffer control.The immunized chickens were challenged with 5x10(4.7) EID50 IBV M41 strain.High antibody responses were detected in chickens immunized with IBV-Flagellin-SAPN.In ex vivo proliferation tests, peripheral mononuclear cells (PBMCs) derived from IBV-Flagellin-SAPN immunized chickens had a significantly higher stimulation index than that of PBMCs from chickens receiving Flagellin-SAPN.
Chickens immunized with IBV-Flagellin-SAPN had a significant reduction of tracheal virus shedding and lesser tracheal lesion scores than did negative control chickens.The data demonstrated that the IBV-Flagellin-SAPN holds promise as a vaccine for IBV.